ʻO ka ʻaila Artemisia capillaris maʻemaʻe no ke kukui a me ke kopa e hana ana i ka diffuser kūʻai nui i ka aila nui hou no nā diffusers puhi ʻohe.

wehewehe pōkole:

Hoʻolālā hoʻohālike rodent

Ua māhele ʻia nā holoholona i ʻelima mau pūʻulu o nā ʻiole he ʻumikumamālima. ʻO ka pūʻulu hoʻomalu a me ka hui pūʻulu hoʻohālike i hoʻopaʻa ʻia meaila sesameno 6 lā. Hoʻopili ʻia nā ʻiole hui mana maikaʻi me nā papa bifendate (BT, 10 mg / kg) no nā lā 6. Ua mālamaʻia nā hui hoʻokolohua me 100 mg / kg a me 50 mg / kg AEO i hoʻoheheʻeʻia i kaʻaila sesame no nā lā 6. Ma ka lā 6, ua mālama ʻia ka pūʻulu mana me ka aila sesame, a ua mālama ʻia nā hui ʻē aʻe a pau me ka nui o 0.2% CCl4 i ka aila sesame (10 ml/kg) e.hoʻokomo ʻia intraperitoneal. A laila hoʻokē ʻai ʻia nā ʻiole me ka wai ʻole, a ʻohi ʻia nā ʻano koko mai nā moku retrobulbar; ʻO ke koko i hōʻiliʻili ʻia ua centrifuged ma 3000 ×gno 10 min e hoʻokaʻawale i ka serum.ʻO ka wehe ʻana o ka ʻāʻīua hana koke ʻia ma hope o ka hemo ʻana o ke koko, a ua wehe koke ʻia nā laʻana o ke ake. Ua mālama koke ʻia kekahi ʻāpana o ke akepaʻa ma −20 ° C a hiki i ka nānā ʻana, a ʻoki ʻia kekahi ʻāpana a hoʻopaʻa ʻia i kahi 10%formalinhoʻonā; ua mālama ʻia nā ʻiʻo i koe ma −80 °C no ka nānā ʻana i ka histopathological (Wang et al., 2008,Hsu et al., 2009,Nie et al., 2015).

ʻO ke ana o nā ʻāpana biochemical i ka serum

Ua helu ʻia ka ʻeha o ke ake ma ka manaʻo ʻana i kanā hana enzymatico ka serum ALT a me AST e hoʻohana ana i nā pahu pāʻoihana e like me nā kuhikuhi no nā kits (Nanjing, Jiangsu Province, Kina). Ua hōʻike ʻia nā hana enzymatic e like me nā ʻāpana no ka lita (U/l).

Ana o MDA, SOD, GSH a me GSH-Pxi loko o ka ate homogenates

Hoʻohui ʻia nā ʻiʻo o ke ake me ka saline physiological anuanu ma kahi ratio 1: 9 (w / v, ate: saline). Ua centrifuged nā homogenates (2500 ×gno 10 min) e hōʻiliʻili i nā supernatants no nā hoʻoholo hope. Ua helu ʻia ka pōʻino o ka ate e like me nā ana hepatic o nā pae MDA a me GSH a me ka SOD a me GSH-P.xnā hana. Ua hoʻoholo ʻia kēia mau mea a pau ma muli o nā ʻōlelo aʻoaʻo ma ka kit (Nanjing, Jiangsu Province, Kina). Ua hōʻike ʻia nā hopena no MDA a me GSH e like me nmol per mg protein (nmol/mg prot), a me nā hana o SOD a me GSH-Pxua hōʻike ʻia ma ke ʻano he U no ka mg protein (U / mg prot).

ʻIkepili histopathological

Ua hoʻopaʻa ʻia nā ʻāpana o ke akepaʻa hou i loko o kahi 10% bufferedparaformaldehydephosphate solution. Hoʻokomo ʻia ka laʻana i loko o ka paraffin, ʻokiʻoki ʻia i loko o nā ʻāpana 3-5 μm, ʻulaʻula me kahematoxylinaeosin(H&E) e like me ke kaʻina hana maʻamau, a hoʻopaʻa hope ʻia emicroscopy māmā(Tian et al., 2012).

Ka helu helu

Ua hōʻike ʻia nā hopena ma ke ʻano he mean ± standard deviation (SD). Ua kālailai ʻia nā hopena me ka hoʻohana ʻana i ka papahana helu SPSS Statistics, version 19.0. Hoʻopili ʻia ka ʻikepili i kahi loiloi o ka ʻokoʻa (ANOVA,p<0.05) a ukaliia e ka ho'āʻo a Dunnett a me ka ho'āʻo T3 o Dunnett e hoʻoholo i nā ʻokoʻa koʻikoʻi ma waena o nā waiwai o nā hui hoʻokolohua like ʻole. Ua noʻonoʻo ʻia kahi ʻokoʻa nui ma kahi pae op< 0.05.

Nā hualoaʻa a me ke kūkākūkā

Nā mea koho o AEO

Ma ka nānā ʻana o GC/MS, ua ʻike ʻia ka AEO he 25 mau mea i hoʻokaʻawale ʻia mai 10 a 35 mau minuke, a ua ʻike ʻia he 21 mau mea nona ka 84% o ka aila pono.Papa 1). ʻO ka ʻaila volatile i loaʻamonoterpenoids(80.9%), sesquiterpenoids (9.5%), saturated unbranched hydrocarbons (4.86%) a me nā ʻano like ʻole acetylene (4.86%). Hoʻohālikelike ʻia me nā haʻawina ʻē aʻe (ʻO Guo et al., 2004), ua loaʻa iā mākou nā monoterpenoids nui (80.90%) ma ka AEO. Ua hōʻike ʻia nā hopena ʻo ka mea nui loa o ka AEO ʻo β-citronellol (16.23%). ʻO nā mea nui ʻē aʻe o AEO he 1,8-cineole (13.9%),camphor(12.59%),linalool(11.33%), α-pinene (7.21%), β-pinene (3.99%),thymol(3.22%), amyrcene(2.02%). ʻO ka hoʻololi ʻana o ka haku melemele e pili ana i nā kūlana kaiapuni i ʻike ʻia ai ka mea kanu, e like me ka wai mineral, ka lā, ka pae o ka ulu ʻana a memeaʻai.

Kumukuai FOB:US $0.5 - 9,999 / ʻāpana

Min.Kauoha Nui:100 ʻāpana/ ʻāpana

Hiki ke hoolako:10000 ʻāpana / ʻāpana i kēlā me kēia mahina

E hoʻouna i ka leka uila iā mākou

Huahana Huahana

ʻO ka maʻi ate, he maʻi maʻamau i hana ʻia emaʻi maʻi hepatitis, ka waiʻona, nā mea ʻawaʻawa i ka ate, nā ʻano meaʻai maikaʻi ʻole a me ka haumia o ke kaiapuni, he mea hopohopo honua ia (Papay et al., 2009). Eia naʻe, paʻakikī pinepine ka mālama ʻana i kēia maʻi a he hopena paʻa. Pākē kuʻunalāʻau lāʻau, ka mea i hoʻohana ʻia no ka mālama ʻana i nā maʻi ate, ke hoʻohana nui ʻia nei e ka poʻe Kina (Zhao et al., 2014).ʻO Artemisia capillarisThunb.,Asteraceae, e like me ka Bencao Gangmu, nā moʻolelo kaulana loa o ka lāʻau lapaʻau kuʻuna Kina, ua hoʻohana nui ʻia ma ke ʻano he lāʻau lapaʻau e hoʻomaʻemaʻe i ka wela, hoʻolaha.diuresisa hoʻopau i ka jaundice a ua hoʻohana ʻia hoʻi i mea ʻono i nā mea inu, nā mea kanu, a me nā pastries no kona ʻala.A. kapiliua manaʻo ʻia he ʻano lāʻau lapaʻau a me nā meaʻai Kina e ka nui o ka poʻe. No laila, ua nui nā hoʻoikaika ʻana e hoʻomohala i nā lāʻau lapaʻau pono, e like meA. kapili, no ka lapaʻau ʻana i ka maʻi ate.

I nā makahiki i hala iho nei, ua loaʻa i nā lāʻau lapaʻau ka nānā a kaulana no ka mālama ʻana i ka maʻi ate ma muli o ko lākou palekana a me ka pono (ʻO Ding et al., 2012).A. kapiliua hōʻike ʻia e loaʻa ka hana hepatoprotective maikaʻi e pili ana i nā ʻano lāʻau lapaʻau hou (Han et al., 2006). He lāʻau lapaʻau koʻikoʻi nō hoʻi ia ma Kina a he anti-inflammatory kaulana (Cha et al., 2009a),choleretic(ʻO Yoon lāua ʻo Kim, 2011), a me ka anti-tumor (Feng et al., 2013)laau lapaau.

Phytochemicalua hōʻike ʻia nā haʻawina he nui o nā aila koʻikoʻi volatile,nā coumarins, aflavonol nā glycosidesa me kahi hui o nā mea i ʻike ʻole ʻiaaglyconesmaiA. kapili(Komiya et al., 1976,Yamahara et al., 1989). ʻO ka ʻaila nui oA. kapili(AEO) ʻo ia kekahi o nā pūhui lāʻau lapaʻau nui a hāʻawi i ka anti-inflammatory (Cha et al., 2009a) a me nā waiwai anti-apoptotic (Cha et al., 2009b). Eia naʻe, ʻoiai ʻo AEO kekahi o nā pūhui nui oA. kapili, nā hana hepatoprotective kūpono o nā mea nui maiA. kapilipono e makaikai.

Ma kēia haʻawina, ka hopena pale o AEO makalapona tetrachloride(CCl4)-i hoʻokomo ʻiahepatotoxicityua loiloi ʻia e nā ʻano biochemical, e like me ka hepatichoemi glutathione(GSH),malondialdehyde(MDA) pae,superoxide dismutase(SOD), aglutathione peroxidase(GSH-Px) hana, a me na hana aaspartate aminotransferase(AST) aalanine aminotransferase(ALT) i ka serum. ʻO ka nui o kaʻeha o ka ate i hoʻokomoʻia e CCl4 ua nānā pūʻia ma o ka nānāʻana i ka histopathological, i hui pūʻia me ka nānāʻana i ka phytochemical e GC-MS eʻike i nā mea o ka AEO.